Study on Interspecific Hybridization of Brassica spp. through in vitro Culture

Authors

  • Chuanpis Aroonrungsikul Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Kasem Piluek Dept. of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Pornpun Phuprompun Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Siriwan Burikum Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.
  • Siriporn Chumsangchotsakul Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Keywords:

interspecific hybridization, Brassica spp., in vitro culture, vernalization

Abstract

The imbibed seed of B. campestris and B. juncea were vernalized for one month and juvenile seedlings of B. oleracea were vernalized for one half month at 5 degree C under 16 light condition, in order to induce flowering. All plants were transferred into the growth cabinet in which controlled 23-25 degree C, continuous light condition. After 20 days cross pollination among species were made, the hybrid capsules were collected and embryos were rescued in the modified MS medium supplemented with casein hydrolysate, BA (0.1 mg/l) and NAA (0.1 mg/l). The embryo of hybrids between B. juncea and B. oleracea; B. campestris and B. juncea; B. campestris and B. oleracea were successfully regenerated to plantlets after 30 days in vitro culture.

Published

1992-12-31

How to Cite

Chuanpis Aroonrungsikul, Kasem Piluek, Pornpun Phuprompun, Siriwan Burikum, and Siriporn Chumsangchotsakul. 1992. “Study on Interspecific Hybridization of Brassica Spp. Through in Vitro Culture”. Agriculture and Natural Resources 26 (5). Bangkok, Thailand:18-24. https://li01.tci-thaijo.org/index.php/anres/article/view/241866.

Issue

Vol. 26 No. 5 (1992): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.