Isolation of Acaricidal Substances against Tropical Cattle Ticks from Sugar Apple Seeds

Authors

  • Narong Chungsamarnyart Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Sugunya Mahatheeranont Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Chainarong Rattankkreetakul Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Suratwadee Jiwajinda Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Weerapol Jansawan Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.

Keywords:

isolation, acaricide, sugar apple seeds, tropical cattle ticks

Abstract

Isolation of acaricidal active substances from sugar apple seeds (Annona squamosa L.) against tropical cattle ticks (Boophilus microplus) was performed. Two active compounds were found in ethyl acetate part of ethanol extracted crude. The compound I (R 0.59) was isolated from twice chromatographed fractions of 60-100% ethyl acetate/toluene (F4-6) and 10, 20% ethanol/ethyl acetate (F7-8), and of 10% ethanol/ethyl acetate (FF 7) by preparative TLC (developed with hexane:acetone; 3:2) and preparative HPLC (reverse phase C subscript 18 column, 85% MeOH in water, 210 nm UV detecter). The compound II (R 0.31) was isolated from triple chromatographed of 80% ethyl acetate/toluene (F5), 45-60% acetone/hexane (F A), and of 40-50% ethyl acetate/toluene (FF B) respectively by preparative TLC (developed with hexane:acetone, 3:2). The compound I and II displayed a highest mass in EI mass spectrum at 623 and 432 mass unit, respectively. The fragmentation pattern of compound I are similar to those known substance; squamocin and acetogenin. The protron NMR spectrum of both compounds displayed the similarly pattern of triterpenoid.

Published

1992-12-31

How to Cite

Narong Chungsamarnyart, Sugunya Mahatheeranont, Chainarong Rattankkreetakul, Suratwadee Jiwajinda, and Weerapol Jansawan. 1992. “Isolation of Acaricidal Substances Against Tropical Cattle Ticks from Sugar Apple Seeds”. Agriculture and Natural Resources 26 (5). Bangkok, Thailand:41-45. https://li01.tci-thaijo.org/index.php/anres/article/view/241870.

Issue

Vol. 26 No. 5 (1992): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.