Effect of Gamma-rays on In vitro culture of Mungbean Cotyledon

Authors

  • Siranut Lamseejan Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Arunee Wongpiyasatid Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Sumin Smutkupt Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Abstract

Multiple shoots were obtained from cotyledons of 7 mungbean cultivars cultured on B5 supplemented with 2, 4, 6 and 8 mg/l BA. Each variety responsed to BA differently. The medium containing 5 mg/l BA was the best for multiple shoot induction. Cotyledon from 3 day-old-seeding gave the highest percentage of shoot multiplication (88.00%) within 18 days. The best medium for root induction was MS medium supplemented with 1 g/l KNO3 and 0.203 mg/l IBA. Seeds of 3 mungbean cultivars were treated with gamma radiation at 200, 400, 600 and 800 Gy and cotyledons were cultured on the medium B5 containing 4 mg/l BA to produce multiple shoots. Shoot and root formations were affected by radiation dose. At 800 Gy, shoot formation was completely inhibited. Rootings were accomplished at radiation dose as high as 400 Gy.

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Published

1991-06-30

How to Cite

Siranut Lamseejan, Arunee Wongpiyasatid, and Sumin Smutkupt. 1991. “Effect of Gamma-Rays on In Vitro Culture of Mungbean Cotyledon”. Agriculture and Natural Resources 25 (2). Bangkok, Thailand:139-45. https://li01.tci-thaijo.org/index.php/anres/article/view/241959.

Issue

Vol. 25 No. 2 (1991): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.