The BHK21C13 Suspension Cells Culture in 5% PEG Treated Serum for FMD Virus and Vaccine Production

Abstract

These experiments were to decrease the amount of PEG treated serum (Bovine serum collecting from slaughter house and treated with 8% PEG 6000) in the medium without changing the character of cultured cells in order to produce high yield of FMD virus and good quality of FMD vaccine. The BHK₂₁C₁₃ suspension cell cultured in the medium containing 5% poly ethylene glycol (PEG) treated serum for 3 days at 37^oC. pH 7.2 The cells were subcultured in the same medium and condition until the 7^th passage. The 7^th passage cells were inoculated by type 0 cattle 0 (OBKK/S1) FMD virus by cell sedimentation method. The inoculated cells were culture in the medium containing 1% PEG treated serum at 37^oC. pH 7.4 and harvested at 18 hours for preparing the vaccines. The results showed that the BHK₂₁C₁₃ suspension cells could grow in the medium containing 5% PEG treated serum, and could produce high yield of FMD virus and good quality of FMD vaccine.