Tissue Culture of Red Kidney Bean

Authors

  • Siranut Lamseejan Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Arunee Wongpiyasatid Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Varaporn Rojanasiriwong Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Abstract

A procedure for regenerating plantlets through organogenesis from tissue culture of red kidney bean was attempted. Seeds of Mecosta cultivar were germinated on MS supplemented with 11.0 mg/l BA. Cotyledonary nodes were excised from 14 day-old-seedling and then cultured on MS to which 0.05, 0.5 and 1 mg/l IAA and NAA in combination with 1.0 and 5 mg/l kinetin and 1.0, 5.0 and 11 mg/l BA were added. Multiple shoot buds were produced only on MS + 0.5 mg/l IAA +11 mg/l BA; MS + 0.5 mg/l NAA + 5 mg/l BA and MS + 1 mg/l NAA +11 mg/l BA. For further shoot development and elongation, shoot buds were cultured on MS, L6 and RB1 containing 1.0 mg/l BA. The highest number of developed shoots were observed on MS while the RB1 yielded the poorest shoot development.

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Published

1991-09-30

How to Cite

Siranut Lamseejan, Arunee Wongpiyasatid, and Varaporn Rojanasiriwong. 1991. “Tissue Culture of Red Kidney Bean”. Agriculture and Natural Resources 25 (3). Bangkok, Thailand:292-98. https://li01.tci-thaijo.org/index.php/anres/article/view/241976.

Issue

Vol. 25 No. 3 (1991): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.