Detection of Sugarcane Mosaic Virus in Sugarcane Plantlets Derived from Meristem Culture

Authors

  • Rongrong Visessuwas Plant tissue culture unit, Central Laboratory and Greenhouse complex, KURDI, Kasetsart University, Nakhon Pathom 73140, Thailand
  • Pissawan Chiemsombat Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Sontichai Chanprame Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Kruik Naritoom Department of Radiation, Faculty of Sciences, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Ratree Kuhapitaktharm Plant tissue culture unit, Central Laboratory and Greenhouse complex, KURDI, Kasetsart University, Nakhon Pathom 73140, Thailand

Abstract

Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2, 4-D including 10% (v/v) coconut water. The plantlets obtained were detected for the presence of sugarcane mosaic virus by using the enzyme-linked immunosorbent assay (ELISA), with alkaline phosphatase and p-nitrophenyl reaction system. Results were evaluated by automatic reading machine and percentage of virus-free stock were determined. It showed that sugarcane plantlets derived from meristem culture provided upto 80-100% virus-free plants which could be mass propagated for further plantation in the field.

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Published

1991-12-31

How to Cite

Rongrong Visessuwas, Pissawan Chiemsombat, Sontichai Chanprame, Kruik Naritoom, and Ratree Kuhapitaktharm. 1991. “Detection of Sugarcane Mosaic Virus in Sugarcane Plantlets Derived from Meristem Culture”. Agriculture and Natural Resources 25 (5). Bangkok, Thailand:1-4. https://li01.tci-thaijo.org/index.php/anres/article/view/242006.

Issue

Vol. 25 No. 5 (1991): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.