Detection of Sugarcane Mosaic Virus in Sugarcane Plantlets Derived from Meristem Culture

Authors

  • Rongrong Visessuwas Plant tissue culture unit, Central Laboratory and Greenhouse complex, KURDI, Kasetsart University, Nakhon Pathom 73140, Thailand
  • Pissawan Chiemsombat Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Sontichai Chanprame Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Kruik Naritoom Department of Radiation, Faculty of Sciences, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Ratree Kuhapitaktharm Plant tissue culture unit, Central Laboratory and Greenhouse complex, KURDI, Kasetsart University, Nakhon Pathom 73140, Thailand

Abstract

Meristem culture of five cultivars of sugarcane, namely, Supan, Nigro, Macos, E-Heudang and CO were cultured on Marashige and Skoog media with 3 mg/l or 1 mg/l 2, 4-D including 10% (v/v) coconut water. The plantlets obtained were detected for the presence of sugarcane mosaic virus by using the enzyme-linked immunosorbent assay (ELISA), with alkaline phosphatase and p-nitrophenyl reaction system. Results were evaluated by automatic reading machine and percentage of virus-free stock were determined. It showed that sugarcane plantlets derived from meristem culture provided upto 80-100% virus-free plants which could be mass propagated for further plantation in the field.

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Published

1991-12-31

How to Cite

Rongrong Visessuwas, Pissawan Chiemsombat, Sontichai Chanprame, Kruik Naritoom, and Ratree Kuhapitaktharm. 1991. “ Detection of Sugarcane Mosaic Virus in Sugarcane Plantlets Derived from Meristem Culture”. Agriculture and Natural Resources 25 (5). Bangkok, Thailand:1-4. https://li01.tci-thaijo.org/index.php/anres/article/view/242006.

Issue

Vol. 25 No. 5 (1991): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.