Effects of CO2 Enrichment and Sucrose Concentrations on Growth of Papaya Shoots Cultured In Vitro

Abstract

Study on growth of papaya shoot variety ‘Ko Ko’ in culture supplemented with different CO₂ concentrations enriched to CO₂ incubator : atmosphere (0.03), 1, 3 and 5%, various concentrations of sucrose added to modified MS medium: 0, 1, 2, and 3%. Two types of bottle covered were used: plastic caps and aluminum foil with membrane filter for gas exchange. The culture were kept in CO₂ incubator under 1,200 lux light intensity, 12 hrs. photoperiod for three weeks. Determination of papaya shoot growth was achieved by calculating percent increment by weight. Papaya shoots cultured in the medium with 1% sucrose and 5% CO₂ enrichment yielded the highest increasing percentage. The result shows that there was no significant difference in weight among sucrose and CO₂ concentrations but there was significant difference among types of the bottle covered.