Molecular Cloning of Restriction Endonuclease Fragments of DNA Isolated from Nuclear Polyhedrosis Virus of Heliothis armigera

Authors

  • Tipwadee Attathom Department of Entomology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Boonnapha Isanont Department of Entomology, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Supat Attathom Plant Genetic Engineering Unit, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand.

Abstract

Restriction fragments of Heliothis armigera nuclear polyhedrosis virus (HaNPV) DNA double digested with EcoRI and BamHI were cloned in E. coli DH  5αF' by using the Bluescript plasmid as a vector. Inserted DNAs were estimated to be 1.5 and 2.0 kb in size. DNA probe was conducted from DNA fragment of 1.5 kb labeled with digoxigenin, the nonradioactive DNA labeling. The probe hybridized well with DNAs isolated from positive colonies but not with the Bluescript plasmid. Dot blot hybridization data indicated that this digoxigenin labeling DNA probe can detect the viral DNA in the infected larva two days after inoculation. This probe can also be used to detect viral DNA isolated from a single NPV infected H. armigera larva.

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Published

1991-12-31

How to Cite

Tipwadee Attathom, Boonnapha Isanont, and Supat Attathom. 1991. “Molecular Cloning of Restriction Endonuclease Fragments of DNA Isolated from Nuclear Polyhedrosis Virus of Heliothis Armigera”. Agriculture and Natural Resources 25 (5). Bangkok, Thailand:21-25. https://li01.tci-thaijo.org/index.php/anres/article/view/242009.

Issue

Vol. 25 No. 5 (1991): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.