Peroxidase Isozyme Activity in Tomato Plants Infected by Tomato Yellow Leaf Curl Virus

Abstract

Plant peroxidases have been purported to play an important role in plant resistance to bacterial, fungal, and viral diseased. The positive correlations were found between the increase in peroxidase activity and disease resistance of plant tissue after infection. The objective of this study was to determine whether peroxidase activity and its isozyme pattern were altered by tomato yellow leaf curl virus (TYLCV) infection. The infection of TYLCV was obtained by feeding viruliferous white flies onto tomato (Lycopersicon esculentum Mill cv. Seeda) plant overnight. Ten to fourteen days after infection, peroxidase enzyme was extracted for leaf tissue and fractionated with 10% polyacrylamide gel electrophoresis. The differences in peroxidase isozyme patterns and activity were found between healthy and TYLCV-infected plants. TYLCV-infected tomato plants constained fewer isozymes but with higher activity than healthy controls. This suggested that peroxidase isozymes could be an indicator of disease infection in tomato.