Rapid Detection of Aflatoxin by Serological Mathods

Authors

  • Pranee Hamelink Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Chintana Chana Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Chiradej Chamswarng Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand
  • Ronnapop Bunjoedchaoedchoo Department of Plant Pathology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand

Abstract

Antiserum was produced by using the following antigens: Aspergillus flavus (AFC), non aflatoxin producing, isolated in Thailand: A. flavus (AFB5), high aflatoxin producing , isolated in Thailand; A. parasiticus (AP 827), high aflatoxin producing, isolated in U.S.A.; Aflatoxin B1 (AF). After collecting normal serum, antigens were prepared and injected into 5 rabbits. Antisera were collected at weekly intervals 10-20 times. Only low titers of antisera could be detected by precipitation reaction on slides. The antisera were not sufficient for use in ELISA. Preliminary tests using ALFA CHECK a product by UBE Industries Ltd, demonstrated clearly the high sensitivity of ELISA for Aflatoxin detection. AFB 1 was detected at concentrations as low as 4 ppb in 20 minutes.

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Published

1991-12-30

How to Cite

Pranee Hamelink, Chintana Chana, Chiradej Chamswarng, and Ronnapop Bunjoedchaoedchoo. 1991. “Rapid Detection of Aflatoxin by Serological Mathods”. Agriculture and Natural Resources 25 (5). Bangkok, Thailand:126-30. https://li01.tci-thaijo.org/index.php/anres/article/view/242022.

Issue

Vol. 25 No. 5 (1991): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.