Antioxidant and acetylcholinesterase inhibitory activities of Morinda citrifolia L. extracts


  • Pornpat Sam-ang Department of Chemistry, Faculty of Science and Technology, Pibulsongkram Rajabhat University, Phitsanulok 65000, Thailand.
  • Damratsamon Surangkul Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000, Thailand.
  • Kongdech Savaspun Department of Chemistry, School of Science, University of Phayao, Phayao 56000, Thailand.
  • Anuchit Phanumartwiwath Chulabhorn Graduate Institute, Chemical Biology Program, Chulabhorn Royal Academy, Lak Si, Bangkok 10210, Thailand.


Acetylcholinesterase, Alzheimer’s disease, Antioxidant activity, Morinda citrifolia L, Total phenolic contents


RESOURCESExtractions of roots, leaves and stems of Morinda citrifolia L. were carried out by maceration using ethanol, 40% ethanol/water and water, respectively. The aqueous leaf extract, ethanolic stem extract and aqueous root extract were obtained with highest percentage yields of 10.92%, 3.92% and 6.37% dry weight, respectively. The bioactivities of all the crude extracts were evaluated regarding their antioxidant and anti-acetylcholinesterase (AChE) activities. The crude ethanolic extract from roots had the relatively highest antioxidant activity with a half maximal inhibitory concentration (IC50) value of 5.97 mg/mL and total phenolic contents of 13.33 mg gallic acid equivalent/g crude extract. Moreover, the ethanolic root extract also had the most potent anti-AChE inhibitory activity with an IC50 value of 454 μg/mL. This study indicated that ethanolic M. citrifolia root extract should be a good candidate for AChE inhibitors which may be applied as herbal supplements for the prevention of Alzheimer’s disease.


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How to Cite

Sam-ang, Pornpat, Damratsamon Surangkul, Kongdech Savaspun, and Anuchit Phanumartwiwath. 2020. “Antioxidant and Acetylcholinesterase Inhibitory Activities of Morinda Citrifolia L. Extracts”. Agriculture and Natural Resources 54 (2). Bangkok, Thailand:173–179.



Research Article