The ovarian structure and oogenesis of the pea crab Pinnotheres cyclinus Gordon, 1932: A histological investigation

Authors

  • Sinlapachai Senarat Department of Marine Science and Environment, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang Campus, Sikao, Trang 92150, Thailand.
  • Lamai Thongboon Department of Biology, Faculty of Science, Prince of Songkla University, Songkhla 90110, Thailand.
  • Jes Kettratad Department of Marine Science, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand.
  • Wannee Jiraungkoorskul Pathology Information and Learning Center, Department of Pathobiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Keywords:

Histology, Microanatomy, Oocyte, Ovary, Thailand

Abstract

The pea crab Pinnotheres cyclinus is a parasite of cultured mollusks. However, the information on reproductive histology of this crab has remained limited. Therefore, the present study investigated the ovarian structure and oogenesis of Pinnotheres cyclinus during maturation using histological techniques. Histologically, the ovary of this crab was found to be surrounded by a thin epithelium and connective tissue of the ovarian wall. Different phases of oogenesis were observed in the germinal area and could be classified into four phases: oogonial proliferation, the primary growth phase, the secondary growth phase, and the atretic oocyte phase. An oogonium was located in the ovarian cyst of the ovarian lobe, which was surrounded by a layer of pre-follicular cells. During the primary growth phase, oogonia continued to develop in the ovarian cyst, accumulating lipids
and cortical alveoli. The appearance of spherical yolk granules related to changes in follicular cells during the secondary growth phase was also observed. These yolk granules reacted positively to Masson’s trichrome and Mollary’s trichrome staining, implying the presence of mucopolysaccharides and glycoproteins. Atretic oocytes were also found. Stages of embryonic development were also observed, including the formation of egg membranes covering embryos. Consequently, a fertilized egg was then filled with yolk granules, which all gradually combine to become one within the egg.

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Published

2020-04-24

Issue

Section

Research Article