Embryogenesis and Plant Regeneration from Immature cotyledon Culture Soybean

Authors

  • Sontichai Chanprame Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140. Thailand.
  • Rachanee Therapotchanart Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140. Thailand.
  • Peerasak Srinives Department of Agronomy, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140. Thailand.

Abstract

Young soybean cotyledons of various sizes were cultured on MS basic medium added with B-5, vitamins, NAA and other growth regulators at various concentrations. It was found that modified MS medium plus 7.5 to 10.0 mg/l NAA and 15 gm/l sucrose induced best embryoid formation. No effect was detected from other growth promoters or amino acid ( such as casein hydrolysate ) used in the experiment. Culture growth responses were also vatietal dependent. It was observed that culture of ‘SJ4’ cotyledon had the highest frequency of embryoid formation.

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Published

1990-09-30

How to Cite

Sontichai Chanprame, Rachanee Therapotchanart, and Peerasak Srinives. 1990. “Embryogenesis and Plant Regeneration from Immature Cotyledon Culture Soybean”. Agriculture and Natural Resources 24 (3). Bangkok, Thailand:332-38. https://li01.tci-thaijo.org/index.php/anres/article/view/242224.

Issue

Vol. 24 No. 3 (1990): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.