Protoplast Isolation Technique of Osmotolerant Yeast

Authors

  • Manee Tantirungkij Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Orawan Anuvongpathom Central Laboratory and Greenhouse Complex, KURDI, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.

Abstract

Protoplasts of osmotolerant yeast strain AM-06 and AM-35 were isolated by digesting cell wall with lytic emzyme mixture containing 10 mg zymolyase-5000, 0.1 M 2-mercaptoethanol and 2/15 M phosphate buffer ( pH 7.5 ) in the presence of 0.6 M and 1.0 M KCl, respectively. The higher yield of protoplast formation was obtained from cells in log phase. In term of osmotic stabilizer, sorbital and mannitol can be used instead of KCl.

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Published

1990-09-30

How to Cite

Manee Tantirungkij, and Orawan Anuvongpathom. 1990. “Protoplast Isolation Technique of Osmotolerant Yeast”. Agriculture and Natural Resources 24 (3). Bangkok, Thailand:339-46. https://li01.tci-thaijo.org/index.php/anres/article/view/242226.

Issue

Vol. 24 No. 3 (1990): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.