Charaterization of Nucleic Acid of Tomato Yellow Leaf Curl

Authors

  • Supat Attathom Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen, Campus, Nakhon Pathom, 73140, Thailand.
  • Pissawan Chiemsombat Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen, Campus, Nakhon Pathom, 73140, Thailand.
  • Thira Sutabutra Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen, Campus, Nakhon Pathom, 73140, Thailand.
  • Raporn Pongpanitanond Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen, Campus, Nakhon Pathom, 73140, Thailand

Abstract

Tomato yellow leaf curl virus (TYLCV) was successfully isolated from infected SVRDC-4 and Seeda tomato plants. Purification procedure consisted of the extraction of powderized infected tissues in tris- HCI buffer, clarification with chloroform of butanol, precipitation of the virus with polyethylene glycol, and virus purification though caecium sulfate centrifugation. The viral nucleic acid was isolated from purified virus preparation by phenol-chloroform extraction followed by ethanol precipitation. Positive reaction to diphenylamine test and sensitivity to DNAse of isolated nucleic acid suggested that it is DNA. Studies on hyperchromic shift of heat and formaldehyde treated preparations indicated that TYLCV-nucleic acid is single stranded DNA. Its molecular weight as estimated by 0.7 – 0.8 % agarose gel electrophoresis is 0.63 x 106 daltons.

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Published

1990-12-31

How to Cite

Supat Attathom, Pissawan Chiemsombat, Thira Sutabutra, and Raporn Pongpanitanond. 1990. “Charaterization of Nucleic Acid of Tomato Yellow Leaf Curl”. Agriculture and Natural Resources 24 (5). Bangkok, Thailand:1-5. https://li01.tci-thaijo.org/index.php/anres/article/view/242252.

Issue

Vol. 24 No. 5 (1990): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.