DNA Probe and Nucleic Acid Hybridization for Plant Virus Detection

Authors

  • Pissawan Chiemsombat Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen Campus, Nakorn Pathom 73140, Thailand.
  • Wichai Kositratana Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen Campus, Nakorn Pathom, 73140, Thailand.
  • Supat Attathom Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen Campus, Nakorn Pathom, 73140, Thailand.
  • Thira Sutabutra Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen Campus, Nakorn Pathom, 73140, Thailand.
  • Nuchnard Sae-aung Plant Genetic Engineering Unit, Kasetsart University, Kamphaengsaen Campus, Nakorn Pathom, 73140, Thailand.

Abstract

The DNA Probe for detection of tomato yellow leaf curl virus (TYLCV) was constructed by using the oligolabelling method. Cloned TYLCV - DNA Component A in a Bluescript vector was linearized by cutiing at EcoRI site, denatured and primed with random hexanucleotide primer. The complementary DNA strand, labeled with 32P, was completely synthesized by Klenow polymerase filling - in reaction. The cDNA probe was denatured and used in virus detection by nucleic acid hybridization technique. The probe obtained had sensitive at 1 picagram level of template DNA, and reacted well with viral DNA either in crude extract or in DNA preparation of infected tomato tissues. The probe can also detect viral DNA from squash blotted samples of infected tissues and viruliferous whitefly vector

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Published

1990-12-31

How to Cite

Pissawan Chiemsombat, Wichai Kositratana, Supat Attathom, Thira Sutabutra, and Nuchnard Sae-aung. 1990. “DNA Probe and Nucleic Acid Hybridization for Plant Virus Detection”. Agriculture and Natural Resources 24 (5). Bangkok, Thailand:12-16. https://li01.tci-thaijo.org/index.php/anres/article/view/242254.

Issue

Vol. 24 No. 5 (1990): January-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.