Determination of Alfatoxins in Mixed Feeds of Poultry Farms with Detection by Minicolumn Chromatiography

Abstract

The mixed feeds were analysed by minicolumn chromatography method, 171 sample of mixed feeds randomly collected from poultry farms in Nakhon-Pathom, Ratchaburi, Chonburi and Chachoengsao provinces during summer season (February-April, 1986-1987), revealed that 38.9% of the sample contained aflatoxins (total aflatoxin B1 + B2) in the range of 20-150 ppb. Another 189 sample of mixed feeds were collected after rainy season (October-December, 1985-1986). The aflatoxin contaimation was found in 125 mixed feed sample (66.5%) at the level in the range of 20-1, 500 ppb. The results of this study indicated that seasonal variation has an influence on animal feeding stuff contamination with aflatoxins and an method for aflatoxin determination with minicolumn chromatography is simple and economical for a rapid screening method. Moreover this method gave the accurate values for aflatoxin at the level of 20 ppb.