Tissue Culture in Rice (Oryza sativa L. : Bamsti 370 variety)

Authors

  • Paderm Ratisoontorn Dept. of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Pradit Pongtongkam Dept. of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Saowanee Suputtitada Dept. of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Supunnee kaensarn Dept. of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Abstract

Seeds of rice (Basmati 370 variety) were cultured on the modified Murashige and Skoog (MS) agar medium (1962) supplemented with 15 percent coconut water and the modified MS medium supplemented with 15 percent coconut water and 1.0, 2.0 mg/l 2,4-dichlorophenoxy acetic acid (2,4 D), 1.0, 2.0 mg/l α-naphthalene acetic acid (NAA) and 0.5, 1.0 mg/l kinetin. It was found that the modified medium supplemented with 15 percent coconut water and the concentration of 2,4 D, NAA and kinetin at 2.0, 2.0 and 0.5 mg/l respectively could induce calli of about 33.33 percent. After subculture in the same medium for 4 weeks calli from these media were transferred to the modified MS agar medium without coconut water and growth regulators, and about 30 percent of them could regenerate to plantles.

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Published

1989-09-30

How to Cite

Paderm Ratisoontorn, Pradit Pongtongkam, Saowanee Suputtitada, and Supunnee kaensarn. 1989. “Tissue Culture in Rice (Oryza Sativa L. : Bamsti 370 Variety)”. Agriculture and Natural Resources 23 (3). Bangkok, Thailand:205-10. https://li01.tci-thaijo.org/index.php/anres/article/view/242296.

Issue

Vol. 23 No. 3 (1989): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.