In vitro Multiple Shoot Formation of Aromatic Rice Seeds

Abstract

Seeds of aromatic rice (Oryza sativa L. cv. Khao Dawk Mali 105) were surface sterilized and cultured on Murashige and Skoog (MS) agar medium supplemented with benzyladenine (BA) or kinetin or 2-isopentenyladenine (2 – iP) at the concentrations of 0, 5, 10, 15, 20, 25 and 30 mg/l to induce multiple shoot formation. It was found that BA was more effective than kinetin and 2 – iP in inducing multiple shoot formation. Among the BA levels tested, BA at a concentration of 25 mg/l induced the highest percentage of multiple shoot – forming seeds and the largest number of shoots per seed, 68% and 10.3 shoots per seed, respectively. Shoots were rooted 100% when transferred to the same medium without growth regulator or supplemented with indoleacetic acid or indolebutyric acid at the concentrations of 0.5 or 1.0 mg/l. The complete plantles could survive when transplanted to soil in pots grown under greenhouse conditions.