A Preliminary Study on Induction of Polyploidy in Clarias batrachus L. by Cold Shock.
Abstract
The two cold shock experiments were conducted in Clarias batrachus L. using temperature of 7±2 degree Celcius. Eggs were shocked 0, 10 and 15 minutes after artificial insemination with the shock duration of 10 and 30 minutes. The results showed that almost every group of shocked eggs vielded tetraploid fry excepted the groups using eggs of 0 and 15 minutes after insemination whit 30 minutes shock duration. The shock duration of 10 minutes gave better hatching and survival rates. The conclusion was drawn that the temperature of 7±2 degree Celcius, although produced tetraploid but led to low survival rates of fry. Attempts should be made in order to get more suitable temperature, and shock duration should be less than 30 minutes.
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