Effects of a-Chlorohydrin on the Reproductive Organs of the Male Dog 1. Histopathological Changes of the Testis and the Initial Segment of Epididymis

Abstract

Sixteen stray dogs were castrated (Hemicastration). The testis and epididymis were examined in order to investigate the normal conditions under the light microscopy. After these dogs were healthy, they were injected with a single dose of a-chlorohydrin (3-chloropropane-1, 2-diol) 100 mglkg b.w. subcutaneously. Testis and epididymis of each couple of the sixteen dogs were removed at different times following the injection-1, 2 and 3 days; 1, 2 and 4 weeks; 2 and 3 months, respectively. The histopathological lesions revealed that the epithelial cells of the seminiferous tubules and the initial segment of epididymis were damaged and degenerated after 1 day of treatment. Tremendous degeneration was observed at 3 days. However, the regeneration of the epithelial cells occurred within 1 week and appeared to be normal phenomena in all dogs after 1 week to 3 months of the experiment. By this preliminary investigation, a single dose of 100 mglkg body wt. of a-chlorohydrin injected subcutaneously had shown to be a temporary inhibition of spermatogenesis.