Feeding Zooplankton by Using High-Protein Unicellular Algae.

Abstract

The study on raising zooplankton by using ten species of high-protein unicellular algae (protein 49.23-64.15%) at the rate of 1 mg/l/day in ten units each of 250 ml beaker having natural water filtered by nylon net cloth (mesh size 120-180 micron). After 2-3 days, six species of the freshwater zooplankton bloomed in the beaker. One from each species was selected and put in 25 ml test tube with 20 ml tap water. Each test tube was adjusted to pH 6.5-9.0 (range 0.1) by H3Po4, CaO, NaHCO3 and Na2 SiO3 (commercial grade), and fed by the same species of high-protein unicellular algae at the rate of 0.5-2 mg/l/day (range 0.05). The results were as follow :- Chlorella sp.(K3) at the rate of 1.35, 1.35, 1.8 and 0.8-0.9 mg/l/day were found suitable for Balantidioides bivacuolata, Stylonychia pustulata, Lepadella benjamini and Rotaria citrinus respectively, and under the media having pH 8.4 adjusted with Na2SiO3, pH 7.6-8.4 with any chemical from CaO, NaHCO3Na2SiO3, pH 8.4-8.5 with CaO, pH 8.4 with Na2SiO3 were also found suitable for these freshwater zooplanktons. The Chlorella sp. (K3) gradually increased from 2 upto 97 mg/l/day within 10 days was found suitable for Moina macrocopa, and pH 8.4 adjusted with any kind of chemicals :- CaO, NaHCO3, Na2SiO3 was also suitable for this species. Mesocyclops leuckarti bloomed very well with chlorella sp. (I) at 2 mg/l/day, with adjusted pH of 8.4-8.6. The using of four species of high-protein unicellular algae at 0.5-2 mg/l/day fed to marine zooplankton at the salinity of 30 ppt. showed that :- Brachionus plicatilis could be bloomed very well with Tetraselmis sp., Chlorella sp. (K3) or Chlorella sp. (149) at 1.35-1.80 mg/l/day. However, Artemia salina could be bloomed with Dunaliella sp. Under the wide concentrations range.