Production of In-house ELISA Test Kit for Detection of Aflatoxin in Agricultural Commodities and Their Validations

Authors

  • Amara Chinaphuti Plant Pathology and Microbiology Division, Department of Agriculture, Chatuchak, Bangkok 10900, Thailand.
  • Chawalert Trikarunasawat Plant Pathology and Microbiology Division, Department of Agriculture, Chatuchak, Bangkok 10900, Thailand.
  • Arunsri Wongurai Plant Pathology and Microbiology Division, Department of Agriculture, Chatuchak, Bangkok 10900, Thailand.
  • Suparat Kositcharoenkul Plant Pathology and Microbiology Division, Department of Agriculture, Chatuchak, Bangkok 10900, Thailand.

Keywords:

aflatoxin, antibody, ELISA test kit, validations

Abstract

A specific microtest plate enzyme immunoassay was developed in Thailand for the rapid detection of aflatoxin B1 (AFB1) using Polyclonal antibody against AFB1 . Percentage of the antibody cross reaction to AFB1, AFB2, AFG1 and AFG2 were 100, 21.4, 25.0 and 2.5% respectively. AFB1-HRP (Horseradish Peroxidase) conjugate was used as direct competitive test for AFB1 detection. The DOA- Aflatoxin ELISA Test Kit was produced for detection of both qualitative and quantitative results within 1 hr after sample preparation with the minimum of 0. 4 ppb per assay. There was no significant difference in detection efficiency of this test kit compared to traditional methods of minicolumn, TLC and HPLC. Moreover, the kit obtained 82-100% AFB1 recovery from the spike samples. Validation for the analysis of AFB1 in sample matrices was also tested using peanut extract which had the virtually identical curves to the standard. Detection efficiency of DOA–Aflatoxin ELISA test kit was not different from those imported from the USA and Italy.

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Published

2002-06-30

How to Cite

Amara Chinaphuti, Chawalert Trikarunasawat, Arunsri Wongurai, and Suparat Kositcharoenkul. 2002. “Production of In-House ELISA Test Kit for Detection of Aflatoxin in Agricultural Commodities and Their Validations”. Agriculture and Natural Resources 36 (2). Bangkok, Thailand:179-86. https://li01.tci-thaijo.org/index.php/anres/article/view/242709.

Issue

Vol. 36 No. 2 (2002): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.