Cloning and Nucleotide Sequence of Four tRNA Genes in Mitochondrial Genome of Thai Walking Catfish, Clarias macrocephalus Günther

Authors

  • Pradit Sangthong Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Amnuay Jondeung Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

nucleotide sequence, tRNA genes, Thai walking catfish, Clarias macrocephalus

Abstract

The Hind III digested fragment of Thai walking catfish mitochondrial DNA was cloned and its nucleotide sequence was determined. This fragment, consisting of 668 bp, was composed of partial WANCY region and COI gene. The partial WANCY region was consisted of partial tRNATrp gene,
complete tRNAAla, tRNAAsn, tRNACys, and tRNATyr genes, and origin of light strand replication (OL). These genes and non-coding sequence (OL) were the same in their organization as those found in other vertebrates. The partial nucleotide sequence of COI stared with GTG and its inferred amino acids were highly conserved as previously described in other fishes. Interestingly, the intergenic spacer between tRNACys and tRNATyr was ten nucleotides in length, which might be unique among Clariid species.

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Published

2003-03-31

How to Cite

Pradit Sangthong, and Amnuay Jondeung. 2003. “Cloning and Nucleotide Sequence of Four TRNA Genes in Mitochondrial Genome of Thai Walking Catfish, Clarias Macrocephalus Günther”. Agriculture and Natural Resources 37 (1). Bangkok, Thailand:33-40. https://li01.tci-thaijo.org/index.php/anres/article/view/242783.

Issue

Vol. 37 No. 1 (2003): January-March

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.