Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay

Authors

  • Montri Punyatong Department of Animal Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Puntipa Pongpiachan Department of Animal Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.
  • Petai Pongpiachan Department of Animal Science, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand.

Keywords:

ochratoxin A, monoclonal antibody, ELISA, mycotoxin, immunoassay

Abstract

Ochratoxin A (OTA), a mycotoxin, is produced from Aspergillus and Penicillium species. It is a nephrotoxin which is very toxic for the kidneys, and normally found in feedstuffs and other feeds. In this study, the monoclonal antibody (MAb) against OTA was produced to determine OTA in feedstuffs. It was found that 3 monoclones (5E7-3F2, 5E7-3G6 and 5E7-G10) reacted with OTA. MAb developed a specific and highly sensitive sandwich ELISA. No difference in quantification in samples was found when compared with a commercial test kit (P>0.05) and 50% binding of standard curve was found at 35 pg/assay. In this study OTA in feedstuff could be detected by MAb, which was self-producing by the ELISA method.

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Published

2003-06-30

How to Cite

Montri Punyatong, Puntipa Pongpiachan, and Petai Pongpiachan. 2003. “Monoclonal Antibodies Production for Quantification of Ochratoxin A in Feedstuffs by Enzyme-Linked Immunosorbent Assay”. Agriculture and Natural Resources 37 (2). Bangkok, Thailand:137-44. https://li01.tci-thaijo.org/index.php/anres/article/view/242854.

Issue

Vol. 37 No. 2 (2003): April-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.