Antimicrobial Activity of Medicinal Plant Extracts against Foodborne Spoilage and Pathogenic Microorganisms

Authors

  • Crisanto Maglaque Lopez Ecole Nationale Supérieure des Industries Agroalimentaires, 34033 Montpellier Cedex 01, France.
  • Sunee Nitisinprasert Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Penkhae Wanchaitanawong Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Ngamtip Poovarodom Department of Packaging Technology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Piper betle Linn., Phyllanthus niruri, antimicrobial substance, minimum inhibitory concentration (MIC)

Abstract

Crude ethanol extracts from dried Phyllanthus niruri (DPN), fresh (FPB) and dried Piper betle Linn. (DPB) were tested for their inhibitory activity against three foodborne pathogenic microorganisms (Escherichia coli, Staphylococcus aureus and Salmonella derby) and five foodborne spoilage
microorganisms (Pseudomonas aeruginosa, Bacillus subtilis, Lactobacillus sp., Saccharomyces cerevisiae and Aspergillus niger). Screening for antimicrobial activity using disc diffusion assay showed the inhibition of all tested microorganisms by DPB. On the other hand, only two of the test microorganisms (S. aureus and Lactobacillus sp.) were inhibited by FPB. Likewise, Lactobacillus sp. was the only test microorganism inhibited by DPN. Minimum inhibitory concentrations (MIC’s) of the extracts were determined using agar dilution method on the same test microorganisms. Extract from DPB gave MIC values ranging from 160 to 10,240 parts per million (ppm). Results showed S. cerevisiae as the most sensitive and B. subtilis as the least sensitive to the extract. Both FPB and DPN extracts did not show growth inhibition of test microorganisms at the highest concentration used (10,240 ppm).

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Published

2003-12-31

How to Cite

Crisanto Maglaque Lopez, Sunee Nitisinprasert, Penkhae Wanchaitanawong, and Ngamtip Poovarodom. 2003. “Antimicrobial Activity of Medicinal Plant Extracts Against Foodborne Spoilage and Pathogenic Microorganisms”. Agriculture and Natural Resources 37 (4). Bangkok, Thailand:460-67. https://li01.tci-thaijo.org/index.php/anres/article/view/242890.

Issue

Vol. 37 No. 4 (2003): October-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.