Synergism and Detoxification Mechanism of Crude Sugar Apple Seed Extract in Tetranychus truncatus Ehara (Prostigmata: Tetranychidae)
Keywords:
Tetranychus truncatus, synergist, detoxification mechanism, sugar apple extractAbstract
Synergism of maleic acid (MA), piperonyl butoxide (PBO) and triphenyl phosphate (TPP) combined with sugar apple seed extract on the mortality of T. truncatus adult females was performed by the spray method. Crude sugar apple hexane extract+0.5% MA induced the highest toxicity to T.
truncatus (LC50=2.27%), followed by crude hexane extract (LC50=3.52%), crude hexane extract+0.2% PBO (LC50=3.79%) and crude hexane extract+0.2% TPP (LC50=4.13%). Tetranychus truncatus mites exhibited similar esterase activity levels (0.09-0.13 n mole/min/mg protein) in all treatments including the control (untreated mites). Mites in the control exhibited relatively higher GST activity (0.32 n mole/min/mg protein) compared to all other treatments (0.14-0.27 n mole/min/mg protein). Both synergism effect and detoxification mechanism suggested that glutathione S-transferase might play an important role in sugar apple seed extract degradation.
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