Cellular Chemo-Resistance and Radiosensitivity of Parental and Adriamycin-Selective Human Small-Cell Lung Cancer Cell Lines

Authors

  • Yanitsa Chantapoon Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Krongchan Ratanaphadit Department of Biotechnology, Faculty of Sciences, Burapha University, Chonburi 20131, Thailand.
  • Somchit Palakas Kasetsart University Nuclear Technology Research Centre (KU-NTRC), Bangkok 10900, Thailand.

Keywords:

cellular radiosensivity, doxorubicin, adriamycin-selective cells, resazurin assay

Abstract

The radiosensitivity of two small-cell, lung cancer cell lines comprising the adriamycin-selective cell line (GLC4/Adr) and its parental counter cell line (GLC4) was investigated in an aqueous soluble system. A simplified resazurin assay was optimized and used in the cell viability investigation. Cell survival was evaluated after being exposed to cytotoxic agents, deduced from the resazurin reduction using the optical density of a spectrophotometer instead of a fluorescent signal. Compared with their parental counterparts, the GLC4/Adr cells displayed a high resistance capability to doxorubicin with a resistant factor of about 320. No significant resistance to gamma radiation was demonstrated after exposure to gamma radiation from a Cs-137 source. The GLC4/Adr cell line responded to radiation in the same manner as its parental counterpart. This finding suggested that a drug resistance phenotype occurred in the GLC4/Adr cells (plausibly acquired after being cultured in the presence of oxidative stress produced by the step-wise concentration of adriamycin) and it is not responsible for the radioresistance of the cells.

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Published

2013-06-30

How to Cite

Chantapoon, Yanitsa, Krongchan Ratanaphadit, and Somchit Palakas. 2013. “Cellular Chemo-Resistance and Radiosensitivity of Parental and Adriamycin-Selective Human Small-Cell Lung Cancer Cell Lines”. Agriculture and Natural Resources 47 (3). Bangkok, Thailand:424-33. https://li01.tci-thaijo.org/index.php/anres/article/view/243083.

Issue

Vol. 47 No. 3 (2013): May-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.