In Vitro Chromosome Doubling in Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] Using Colchicine and Oryzalin

Authors

  • Surawit Wannakrairoj Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Wondyifraw Tefera Plant Biotechnology Division, Jimma Agricultural Research Center (JARC), Ethiopian Institute of Agricultural Research (EIAR), P.O. Box 1161, Jimma, Ethiopia.

Keywords:

polyploid, mixoploid, cytochimera, indexing

Abstract

Ploidy manipulation holds high promise for the improvement of korarima (Aframomum corrorima) plants with altered morphology, as well as of potentially altered essential oil yields and oil composition. The present work aimed to establish a protocol for in vitro polyploid induction of korarima. Nodal explants 2 mm long were cultured in liquid media with 0, 125, 250, 375 or 500 µM colchicine or 10, 20, 40, 60 or 80 µM oryzalin for 7 d. The lowest concentration was the most effective for polyploid induction with both colchicine and oryzalin. Teteraploid plants were obtained only from shoots regenerated from explants treated with 125 µM colchicine and 10 µM oryzalin. Oryzalin was thus confirmed to be effective at a lower concentration than colchicine.

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Published

2013-10-31

How to Cite

Wannakrairoj, Surawit, and Wondyifraw Tefera. 2013. “In Vitro Chromosome Doubling in Korarima [Aframomum Corrorima (Braun) P.C.M. Jansen] Using Colchicine and Oryzalin”. Agriculture and Natural Resources 47 (5). Bangkok, Thailand:684-94. https://li01.tci-thaijo.org/index.php/anres/article/view/243117.

Issue

Vol. 47 No. 5 (2013): September-October

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.