A Proteomic Approach to Analyze Rice Bran and Shoots of Kao Dawk Mali 105 and its Mutants

Authors

  • Arunee Trisiriroj King Mongkut’s University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140, Thailand.
  • Shui-Tein Chen Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan R.O.C
  • Narumon Jeyashoke King Mongkut’s University of Technology Thonburi, Bangmod, Toongkru, Bangkok 10140, Thailand.

Keywords:

proteomic, Orysa sativa L. ssp. indica, Kao Dawk Mali 105

Abstract

Proteomic analysis was used to investigate bran proteins from wild-type Oryza sativa L. variety KDML105 and its mutant RD15. Fractionation of bran proteins by sequential solubilization showed about 1,000 total spots in 2-D gel. The 2-D gels revealed the similarity in protein patterns between KDML105 and RD15 because most expressed proteins in bran were house-keeping proteins. Consequently, rice shoots were investigated using etiolated shoots and green shoots of KDML105 and its two mutants, namely RD15 and RD6. Rice shoot proteins were extracted by 10% TCA in acetone. 2-D gels presented a greater number of etiolated proteins than green shoot proteins in all 3 varieties. Due to low expression of RuBisCO enzyme in etiolate, some proteins appeared in higher intensities in etiolated samples than in green shoot samples. As a consequence the proteins were excised for MS analysis. However, most of the different proteins among these three varieties appeared in low intensities. The result of this study revealed
that KDML105, RD15 and RD6 expressed different types of salt-stress induced proteins.

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Published

2004-08-30

How to Cite

Arunee Trisiriroj, Shui-Tein Chen, and Narumon Jeyashoke. 2004. “A Proteomic Approach to Analyze Rice Bran and Shoots of Kao Dawk Mali 105 and Its Mutants”. Agriculture and Natural Resources 38 (4). Bangkok, Thailand:493-500. https://li01.tci-thaijo.org/index.php/anres/article/view/243198.

Issue

Vol. 38 No. 4 (2004): July-August

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.