Prevalence of Flavobacterium psychrophilum Infection in Ayu (Plecoglossus altivelis) in Gunma Prefecture, Japan and Comparison of the gyr B Sequences of Isolates

Authors

  • Hajime Arai Gunma Prefectural Institute of Fisheries, 13 Shikishima, Maebashi, Gunma 371-0036, Japan.
  • Yukio Morita Gunma Prefecture Science and Technology Promotion Office, 1-1-1 Ote, Maebashi, Gunma 371-8570, Japan
  • Kunihiro Nobusawa Gunma Prefectural Institute of Fisheries, 13 Shikishima, Maebashi, Gunma 371-0036, Japan.
  • Masanao Arai Gunma Prefectural Institute of Fisheries, 13 Shikishima, Maebashi, Gunma 371-0036, Japan.
  • Sumalee Boonmar Department of Microbiology and Immunology, Kasetsart University, Bangkok 10900, Thailand.
  • Hirokazu Kimura Gunma Prefectural Institute of Public Health and Environmental Sciences, 378 Kamioki, Maebashi, Gunma 371-0052, Japan.

Keywords:

ayu, Flavobacterium psychrophilum, gyr B, sequence analysis

Abstract

The prevalence of cold-water disease (CWD) in ayu (Plecoglossus altivelis) caused by Flavobacterium psychrophilum was investigated in 3 different sites along the Tone River in Gunma Prefecture, Japan. F. psychrophilum was isolated from 7 of 8 fishes from site A (88%), 15 of 101 from site B (15%), and 3 of 8 from site C (38%). The gene encoding DNA gyrase subunit B (gyrB) of F. psychrophilum in 16 isolates was partially sequenced. Among the 16 strains, 15 strains had gene sequences that completely matched those of a species type strain (NCIMB1947T). Another strain confirmed one nucleotide substitution, however, the codon resulting from this substitution was synonymous with that in the type strain sequence. The results suggested that CWD was widespread in the Tone River of Gunma Prefecture, with little genetic divergence from the species type strain.

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Published

2004-08-30

How to Cite

Hajime Arai, Yukio Morita, Kunihiro Nobusawa, Masanao Arai, Sumalee Boonmar, and Hirokazu Kimura. 2004. “Prevalence of Flavobacterium Psychrophilum Infection in Ayu (Plecoglossus Altivelis) in Gunma Prefecture, Japan and Comparison of the Gyr B Sequences of Isolates”. Agriculture and Natural Resources 38 (4). Bangkok, Thailand:523-30. https://li01.tci-thaijo.org/index.php/anres/article/view/243202.

Issue

Vol. 38 No. 4 (2004): July-August

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.