A Chromatographic and Electrophoretic Study of Hemoglobin of Domestic Fowl

Authors

  • Raksak Sittivilai Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Choosri Sribhen Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Supaporn Isariyodom Department of Animal Science, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Thaweesak Songserm Department of Pathology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Apassara Choothesa Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

hemoglobin, chromatography, electrophoresis, domestic fowl

Abstract

Gel filtration column chromatography and sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE) were used to study the chemical properties of hemoglobins for 2-week-old broilers, adult broilers and adult layer hen as well as adult ducks. All of the elution patterns obtained with 0.05 M Tris buffer pH 7.4 containing 0.02% sodium azide and monitored at 415 nm for hemoglobin, was consisted of one peak which was estimated as a molecular weight of 51,784 daltons. As compared with the result from SDS-PAGE, the denatured hemoglobin migrated into two bands with the molecular weight of 12,519 daltons and 10,984 daltons.

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Published

2004-12-30

How to Cite

Raksak Sittivilai, Choosri Sribhen, Supaporn Isariyodom, Thaweesak Songserm, and Apassara Choothesa. 2004. “A Chromatographic and Electrophoretic Study of Hemoglobin of Domestic Fowl”. Agriculture and Natural Resources 38 (6). Bangkok, Thailand:132-36. https://li01.tci-thaijo.org/index.php/anres/article/view/243224.

Issue

Vol. 38 No. 6 (2004): November-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.