Light microscopic Identification Type II and Type III Acini Cells of Salivary Gland of Tropical Cattle Ticks (Boophilus microplus)

Abstract

Light microscopic study the cell types in the acinus of salivary gland of tropical cattle tick (Boophilus microplus). The result showed that six different types of cells can be identified in thicksections stained with Methylene blue Azure II - Basic fuchsin. The identification of the cells was based on the difference in staining of their granules as well as their morphology of the cells. In type II acinus ‘a’ cells containing granules which were spherical, packed tight together and stained in deep blue whereas ‘b’ cells had a less granules than ‘c’ cells, their granules appeared in loosely pattern and most of granules were stained in blue and some in pink. The ‘c’ cells were classified into two types, granular and agranular cells. The granular ‘c’ cell, contained pink and blue granules. In type III acinus ‘d’ cell had granules which were similar to those of ‘a’ cells. Granules of ‘e’ cells were larger than those of ‘d’ cell and stained in blue to light blue and ‘f ‘cell were agranular cells.