Genetic Diversity of Andrographis paniculataWall. ex Nees as Revealed by Morphological Characters and Molecular Markers

Authors

  • Tathaporn Maison Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Hugo Volkaert NSTDA-BIOTEC Thailand Science Park, 113 Phaholyothin Road, Klong Luang, Pathumthani 12100, Thailand.
  • Unaroj Boonprakob Department of Horticulture, Faculty of Agriculture, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
  • Yingyong Paisooksantivatana Department of Horticulture, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Andrographis paniculata, diversity, molecular marker

Abstract

Intraspecific variation of twenty-five accessions of Andrographis paniculata Wall. ex Nees, collected from different geographical areas in Thailand and one from Laos, was determined by morphological characters and molecular analysis using RAPD and SSCP techniques. Morphological
characters and total lactone content variation were measured at 50% flowering time among the accessions grown under uniform environmental condition. UPGMA cluster analysis of all accessions resulted in 4 major groups based on 18 morphological characters while RAPD analysis distinguished 5 groups at 0.82 of similarity coefficient, using 247 bands generated from 14 primer amplifications separated on polyacrylamide gel. Morphological characters and RAPD were incongruent and were not correlated to geographical area of collection and yield of active compound. SSCP analysis showed little polymorphism of specific amplified products. Markers for the CPS1-2, IDH1, IDH2 and IPI genes were monomorphic
and only 2 alleles were detected for CAT and CPS1-1 genes.

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Published

2005-09-30

How to Cite

Tathaporn Maison, Hugo Volkaert, Unaroj Boonprakob, and Yingyong Paisooksantivatana. 2005. “Genetic Diversity of Andrographis PaniculataWall. Ex Nees As Revealed by Morphological Characters and Molecular Markers”. Agriculture and Natural Resources 39 (3). Bangkok, Thailand:388-99. https://li01.tci-thaijo.org/index.php/anres/article/view/243337.

Issue

Vol. 39 No. 3 (2005): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.