Genetic Diagnosis of Sex and Trisomies 13, 18, 21 in Human Single Cell Embryo by Multiplex Fluorescent Polymerase Chain Reaction

Authors

  • Anna Wongkularb Department of Obstetrics&Gynecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
  • Budsaba Rerkamnuaychoke Department of Pathology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
  • Sawaek Weerakiet Department of Obstetrics&Gynecology, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
  • Somphop Navephap Department of Zoology, Faculty of Science, Kasetsart University,Bangkok 10900, Thailand.
  • Amara Campiranon Department of Genetics, Faculty of Science, Kasetsart University,Bangkok 10900, Thailand.

Keywords:

aneuploidy, multiplex fluorescent PCR, PGD

Abstract

A multiplex fluorescent PCR was developed for preimplantation genetic diagnosis (PGD) to investigate aneuploidy screening of chromosomes 13, 18, 21, X and Y in a single human blastomere. The multiplex fluorescent PCR was able to give successful amplification in 30 cases. This technique was
found to be sufficiently sensitive to reveal the peaks of trisomic alleles in 20 pg DNA of control samples. Multiplex fluorescent PCR products of short tandem repeats (STRs) located on chromosomes 13, 18, 21, X and Y from a single human blastomere in a single tube have recently been used for the detection of common chromosome aneuploidies in a single human blastomere.

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Published

2005-09-30

How to Cite

Anna Wongkularb, Budsaba Rerkamnuaychoke, Sawaek Weerakiet, Somphop Navephap, and Amara Campiranon. 2005. “Genetic Diagnosis of Sex and Trisomies 13, 18, 21 in Human Single Cell Embryo by Multiplex Fluorescent Polymerase Chain Reaction”. Agriculture and Natural Resources 39 (3). Bangkok, Thailand:440-45. https://li01.tci-thaijo.org/index.php/anres/article/view/243344.

Issue

Vol. 39 No. 3 (2005): July-September

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.