Stomatal Size, Stomatal Frequency and Pollen Grain Diameter as Indirect Method for Identification of Ploidy Levels in Cotton

Authors

  • Arunee Wongpiyasatid Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Praparat Hormchan Department of Entomology, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.
  • Katarut Chusreeaeom Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Ngamchuen Ratanadilok Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

cotton, Gossypium arboreum, colchicine, polyploidy, stomatal size

Abstract

Seeds of diploid local variety cotton (Gossypium arboreum), PM2, were treated with 0, 0.1 and 0.5% colchicine solutions at duration of 24 and 36 hours for polyploidy induction. Seed germination, seedling survival, stomatal size, stomatal frequency, percent pollen grain sterility and pollen grain diameter were recorded. The results showed seed germination, seedling survial and stomatal frequency to be negatively correlated with colchicine concentrations and durations of seed soaking while numbers of plant having large stomatal size and % pollen grain sterility have positive correlation. Stomatal size increased whereas stomatal frequency decreased. No correlation between pollen grain diameter and colchicine concentrations as well as durations of seed soaking was found.

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Published

2005-12-31

How to Cite

Arunee Wongpiyasatid, Praparat Hormchan, Katarut Chusreeaeom, and Ngamchuen Ratanadilok. 2005. “Stomatal Size, Stomatal Frequency and Pollen Grain Diameter As Indirect Method for Identification of Ploidy Levels in Cotton”. Agriculture and Natural Resources 39 (4). Bangkok, Thailand:552-59. https://li01.tci-thaijo.org/index.php/anres/article/view/243398.

Issue

Section

Research Article