Inducing Salt Tolerance in Purple Guinea Grass (Panicum maximum TD58) via Gamma Irradiation and Tissue Culture

Authors

  • Pradit Pongtongkam Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Sumol Nilratnisakorn Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Surin Piyachoknakul Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Amara Thongpan Department of General Science, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Jantakarn Aranananth Seed and Forage Analysis Section, Animal Nutrition Division, Department of Livestock Development, Bangkok 12000, Thailand.
  • Krisana Kowitwanich Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Sayan Tadsri Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Panicum maximum, gamma ray, tissue culture, salt tolerance

Abstract

The seeds of Panicum maximum TD58 were cultured on MS medium supplemented with 15% coconut water, 5 mg/l kinetin and 100 mg/l casein hydrolysate to induce multiple shoots. The gamma irradiated shoots grown in 0–2.0 % NaCl gave 58 clones of purple guinea grass. All clones were transferred to grow in the natural field. Ten clones of grass with good morphological properties were selected and subsequently grown in the salt stress environment at Maha Sarakham province, Northeastern Thailand. Under salt stress condition, seven out of ten selected clones showed salt tolerant trait. However, there was no difference in AFLP fingerprinting patterns found as compared with the controlled non-irradiated guinea grass. The salt tolerance character might be due to the mutation at a certain location or on a specific gene which could not be distinctly detected by the available AFLP primers.

Downloads

Download data is not yet available.

Downloads

Published

2005-12-31

How to Cite

Pradit Pongtongkam, Sumol Nilratnisakorn, Surin Piyachoknakul, Amara Thongpan, Jantakarn Aranananth, Krisana Kowitwanich, and Sayan Tadsri. 2005. “Inducing Salt Tolerance in Purple Guinea Grass (Panicum Maximum TD58) via Gamma Irradiation and Tissue Culture”. Agriculture and Natural Resources 39 (4). Bangkok, Thailand:681-88. https://li01.tci-thaijo.org/index.php/anres/article/view/243433.

Issue

Section

Research Article