Isolation of Drug Resistant and Enterotoxin Producing Staphylococcus aureus from Cafeterial Foods in Hospitals in Bangkok

Abstract

               The 525 food samples were examined for enterotoxin producing and drug resistance strains of Staphylococcus aureus. The 277 food samples from 12 government hospitals and 248 food samples from 12 private hospitals were examined. The 132 isolates of Staphylococcus aureus were found from 53 (19.13%) food samples of government hospitals and also the 100 isolates were found from 41 (16.53%) food samples of private hospitals. Two hundred and twenty six out of 232 isolates of Staphylococcus aureus were coagulase positive and 74 out of 226 coagulase positive strains could produce enterotoxin. The types of enterotoxin produced by these 74 isolates were A,B,C,A&B and A&C of 13,28,23,4 and 6 isolates, respectively.

                The detection for drug resistance strains of 74 isolates that produced enterotoxin was doned by agar disc diffusion of Kirby-Bauer sensitivity test. The eight kinds of antibiotics and sulfonamides were selected base on the machanism of drugs, they were oxacillin, ciprofloxacin, rifampicin, vancomycin, gentamicin, tetracycline, chloramphenicol and co-trimoxazole. It was found that all isolates were sensitive to oxacillin,ciprofloxacin and rifampin (100%). Some were resisted to tetracycline (43.24%), chloramphenicol (17.57%) and gentamicin (4.05%), a few were intermediate to chloramphenicol, vancomycin and co-trimoxazole (trimethoprim/sulfamethoxazole). The multidrug-resistance strains were
not found in this study.