Different Growth Media and Growth Phases Affecting on Spray Drying and Freeze Drying of Lactobacillus reuteri KUB-AC5

Abstract

To develop a probiotics adjunct, spray drying and freeze drying of Lactobacillus reuteri KUBAC5 grown in different growth media and growth phases were determined. After cultivation, the viable cell of culture in Basal medium was approximately 1 log cycle lower than that in MRS broth. The
highest survival rate after spray drying (92.5%) was obtained from the culture grown in Basal medium for 12 h while the cultivation of culture in MRS broth for 12 h gave the highest survival rate after freeze drying (86.7%). Lb. reuteri KUB-AC5 at stationary phase was more resistant to stress of drying than exponential phase cultures. It was found that the ability of dried cell to produce antimicrobial substances was not affected by drying processes and storage. During storage at 4°C for 2 months, the viable cell of spray dried cells decreased approximately 1 log cycle while the viable cell of the freeze dried cells were quite stable.