Biochemical Properties of Nile Tilapia (Oreochromis niloticus) Hemoglobin

Authors

  • Kriangkrai Thongsarn Center of Agricultural Biotechnology, Interdisciplinary Graduate Program, Kasetsart University, Kamphaengsaen 73140, Thailand.
  • Wanchai Worawattanamateekul Department of Fishery Products, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Suriyan Tunkijjanukij Department of Marine Science, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Choosri Sribhen Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
  • Apassara Choothesa Department of Physiology, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Nile Tilapia, fish hemoglobin, Tilapia hemoglobin

Abstract

Hemoglobins of O. niloticus (averaged standard length of 18.07 ± 0.89 cm and weight of 216.86 ± 27.13 g) were analysed of some biochemical properties. Average hematocrits were 25.80 ± 3.71% and average hemoglobin concentrations were 7.05 ± 1.08 g/dl. It was found that there were 9 – 11 types of hemoglobin by cellulose acetate electrophoresis. The anodic and cathodic hemoglobins were revealed by native polyacrylamide gel electrophoresis with approximate amounts of 45.72 and 54.28% of total hemoglobin, respectively. In addition, the cathodic hemoglobin was classified by cellulose acetate electrophoresis as cathodic I and cathodic II with approximate amounts of 47.62 and 6.66% of total hemoglobin, respectively. Mass spectrometry was performed to determine molecular weights of hemoglobin. It was found that the molecular weights of native hemoglobin was 63.0 kDa and the molecular weights of subunits were 15.3, 15.6 and 16.1 kDa. The pIs of anodic and cathodic hemoglobins ranged
5.28 – 6.80 and 7.27 – 9.71 , respectively.

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Published

2006-02-28

How to Cite

Kriangkrai Thongsarn, Wanchai Worawattanamateekul, Suriyan Tunkijjanukij, Choosri Sribhen, and Apassara Choothesa. 2006. “Biochemical Properties of Nile Tilapia (Oreochromis Niloticus) Hemoglobin”. Agriculture and Natural Resources 40 (1). Bangkok, Thailand:69-73. https://li01.tci-thaijo.org/index.php/anres/article/view/243506.

Issue

Vol. 40 No. 1 (2006): January-February

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.