Development of Catalase Gene Nuclear DNA-Based Marker for Population Genetic Analysis in Thai Teak (Tectona grandis L.f.)

Authors

  • Jongkon Cheua-ngam Center for Agricultural Biotechnology, Kasetsart University, Kamphaengsaen Campus, Nakhon Pathom 73140, Thailand.
  • Hugo Volkaert National Science and Technology Development Center (NSTDA-BIOTEC), Science Park, KlongLuang, Pathumthani 12120, Thailand.

Keywords:

Catalase, teak, Tectona grandis, population genetics, SSCP

Abstract

The teak Catalase gene was amplified and cloned using consensus PCR primers designed based on sequences from other plant species. The obtained teak Cat DNA sequences were used to develop specific primers. The specific primer set could successfully amplify a specific DNA fragment from teak in all populations studied. Using PCR-SSCP, 5 different alleles were detected. The tested nuclear gene primer set had considerable potential as a DNA marker for population analysis in teak. This approach could be used to specifically amplify fragments in other plant species and applied to study evolution, population genetics, outcrossing rate and mating patterns.

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Published

2006-02-28

How to Cite

Jongkon Cheua-ngam, and Hugo Volkaert. 2006. “Development of Catalase Gene Nuclear DNA-Based Marker for Population Genetic Analysis in Thai Teak (Tectona Grandis L.F.)”. Agriculture and Natural Resources 40 (1). Bangkok, Thailand:91-98. https://li01.tci-thaijo.org/index.php/anres/article/view/243537.

Issue

Vol. 40 No. 1 (2006): January-February

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.