Analysis of Intersectional Hybrids of Dendrobium by RAPD Technique

Authors

  • Sirilak Inthawong Development of Economic Flower Crop : Orchid (DEFCO) Project, Faculty of Agriculture, Chiang Mai University, Chiangmai 50200, Thailand.
  • Weenun Bundithya Department of Horticulture, Faculty of Agriculture, Chiang Mai University, Chiangmai 50200, Thailand.
  • Nuttha Kuanprasert Department of Horticulture, Faculty of Agriculture, Chiang Mai University, Chiangmai 50200, Thailand.
  • Pimchai Apavatjrut Department of Horticulture, Faculty of Agriculture, Chiang Mai University, Chiangmai 50200, Thailand.

Keywords:

Dendrobium, interspecific hybridization, RAPD technique

Abstract

Thirty interspecific hybridizations of inter- or intra-section of Dendrobium were made. Five crosses showed good compatibility and could yield hybrids as follows: 2 intersectional hybridizations, 1) section Phalaenanthe: Den. phalaenopsis (D017) × section Formosae: Den. draconis (D022) and
2) section Dendrobium: Den. finlayanum (D030) × section Formosae: Den. cariniferum (D018); and 3 intrasectional hybridizations, 1) section Dendrobium: D030 × Den. nobile (D031), 2) section Formosae: Den. trigonopus (D037) × D022, and 3) D037 × Den. infundibulum (D034). The greatest number of plantlets (1,250 plantlets) was obtained from cross D037 × D022 whereas the least number (3 plantlets) was found in cross D037 × D034. After transplanting for 6 months, survival rate of D037 × D034 was 100% whereas no survival plant was found in cross D030 × D018. Parents and hybrids of 5 crosses were analyzed by RAPD using 21 decamer primers and the number of primers generating polymorphic patterns of each cross varied. RAPD markers were able to differentiate parents in inter- and intra-sectional crosses and these markers could confirm the interspecific
hybrids.

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Published

2006-04-30

How to Cite

Sirilak Inthawong, Weenun Bundithya, Nuttha Kuanprasert, and Pimchai Apavatjrut. 2006. “Analysis of Intersectional Hybrids of Dendrobium by RAPD Technique”. Agriculture and Natural Resources 40 (2). Bangkok, Thailand:456-61. https://li01.tci-thaijo.org/index.php/anres/article/view/243689.

Issue

Vol. 40 No. 2 (2006): March-April

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.