Competition on Using Nutrient for Growth between Bacillus spp. and Vibrio harveyi

Authors

  • Watchariya Purivirojkul Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Nontawith Areechon Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Prapansak Srisapoome Department of Aquaculture, Faculty of Fisheries, Kasetsart University, Bangkok 10900, Thailand.
  • Monchan Maketon Department of Zoology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

competition, nutrient, Bacillus spp., Vibrio harveyi

Abstract

Competition, by using nutrient for growth, between Bacillus pumilus NW01, B. sphaericus NW02 and B. subtilis NW03 and Vibrio harveyi in vitro was studied by culturing each bacteria in Nutrient Broth (+ 1.5% NaCl). The initial concentration of 102 CFU/ml in monoculture and co-culture
of Bacillus spp. and V. harveyi was used. Total Bacillus and Vibrio counts were conducted after 0, 24, 48, 72, 96 and 120 hours. B. pumilus NW01, B. sphaericus NW02 and B. subtilis NW03 decreased V. harveyi by 39.10, 43.62 and 34.46%, respectively. Antagonistic properties of Bacillus spp. against V. harveyi in vivo was tested by feeding shrimp with spores (1011-1012 CFU/g) of each Bacillus and their mixture at 5 g/kg for 1 month. The amount of Vibrio spp. in the intestine of all Bacillus treated shrimp decreased by 20.97-32.45 % as compared with the control. The results showed that these Bacillus spp. could be applied as an effective probiotic in Penaeus monodon culture.

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Published

2006-04-30

How to Cite

Watchariya Purivirojkul, Nontawith Areechon, Prapansak Srisapoome, and Monchan Maketon. 2006. “Competition on Using Nutrient for Growth Between Bacillus Spp. And Vibrio Harveyi”. Agriculture and Natural Resources 40 (2). Bangkok, Thailand:499-506. https://li01.tci-thaijo.org/index.php/anres/article/view/243697.

Issue

Vol. 40 No. 2 (2006): March-April

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.