Sequence-Tagged Site of Defense-Related Genes for Resistant/ Susceptible Eucalypt Selection to Cryptosporiopsis eucalypti

Authors

  • Yuttana Singchada Center for Agricultural Biotechnology, Kasetsart University, KamphaengSaen Campus, Nakhon Pathom 73140, Thailand.
  • Saowanee Suputtitada Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Poonpilai Suwanarit Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Somsak Apisitwanich Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Eucalyptus, Cryptosporiopsis eucalypti, leaf spot, DNA marker

Abstract

Leaf spot and shoot blight caused by the fungal pathogen, Cryptosporiopsis eucalypti, is the most severe disease for eucalypt plantation in Thailand. The objective of this study was to create a set of DNA markers for resistant clone detection. Twelve clones of eucalypt were selected and screened for disease resistance using intact seedling inoculation and detached leaf techniques in the greenhouse and laboratory. Six clones, SF01, SF06, SF18, SF36, SF94 and SF98, were classified as resistance while the other six, SF03, SF07, SF14, SF16, SF70 and SF86, were susceptible clones. The sequence-tagged-site (STS) marker of pathogenesis-related (PR) genes, plant disease resistance (R) genes, and defense-related genes were analyzed in both DNAs of resistant and susceptible groups using polymerase chain reaction (PCR). Amplified DNA fragments were separated by agarose gel and polyacrylamide gel electrophoresis. The results showed that six markers (Ce1, Ce2, Ce3, Ce4, Ce5 and Ce6) from thirty five primer pairs could be used for susceptible and resistant detection of eucalypt.

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Published

2006-06-30

How to Cite

Yuttana Singchada, Saowanee Suputtitada, Poonpilai Suwanarit, and Somsak Apisitwanich. 2006. “Sequence-Tagged Site of Defense-Related Genes for Resistant/ Susceptible Eucalypt Selection to Cryptosporiopsis Eucalypti”. Agriculture and Natural Resources 40 (3). Bangkok, Thailand:748-54. https://li01.tci-thaijo.org/index.php/anres/article/view/243772.

Issue

Vol. 40 No. 3 (2006): May-June

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.