Acid and Bile Tolerance of Lactobacillus thermotolerans, a Novel Species Isolated from Chicken Feces

Authors

  • Piyanuch Boonkumklao Department of Biology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand.
  • Parichart Kongthong Department of Biology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand.
  • Apinya Assavanig Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Keywords:

Lactobacillus thermotolerans, probiotc, bile, acid tolerance

Abstract

Five strains of a novel thermotolerant lactic acid bacterium, Lactobacillus thermotolerans, isolated from chicken feces were assayed for acid and bile tolerance. Cultures of L. thermotolerans were grown anaerobically at 42°C in four different conditions, Man-Rogosa-Sharpe (MRS) broth at pH
6.5, MRS broth at pH 3.0, MRS broth supplemented with 0.3% (w/v) ox gall and MRS broth supplemented with 7 mM sodium taurocholate, respectively. All strains showed an acid tolerance when incubated at pH 3.0 over a period of 4 h. L. thermotolerans demonstrated significant changes of absorbance (p<0.05) from the control when cultured with 0.3% bile concentration. Strain JCM11427 seemed to show the highest tolerance to bile. However, statistical analyses revealed no significant differences. The conjugated bile salt (sodium taurocholate) at physiological concentration (7 mM) had no effect on growth of the bacterial strains.

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Published

2006-10-30

How to Cite

Piyanuch Boonkumklao, Parichart Kongthong, and Apinya Assavanig. 2006. “Acid and Bile Tolerance of Lactobacillus Thermotolerans, a Novel Species Isolated from Chicken Feces”. Agriculture and Natural Resources 40 (5). Bangkok, Thailand:13-17. https://li01.tci-thaijo.org/index.php/anres/article/view/243872.

Issue

Vol. 40 No. 5 (2006): September-October

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.