Antibody-Binding Motif of Mimetic Peptides to V. cholerae O139 Lipopolysaccharide

Authors

  • Aree Thattiyaphong Interdisciplinary Bioscience Program, Kasetsart University, Bangkok 10900, Thailand.
  • Amornrat Promboon Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Chaivat Kittigul Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Krongkaew Supawat National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000, Thailand.
  • Pathom Sawanpunyalert National Institute of Health, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, 11000, Thailand.
  • Sunanta Ratanapo Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

Vibrio cholerae O139, lipopolysaccharide, random peptide library, FliTrx random peptide

Abstract

This study explores deduced amino acid sequences of mimetic peptides of Vibrio cholerae O139 epitopes in order to design specific antigens for use in diagnostic method. Mimetic peptides expressed on E. coli flagella were selected from a FliTrx random peptide library via the interaction with
purified monoclonal antibody to V. cholerae O139. Inserted nucleotides encoding bound peptides were determined by PCR. Peptides from clones giving positive results were confirmed by Western blot analysis. Sixty-two positive E. coli colonies were obtained and nucleotide-sequenced. Inserted nucleotides were translated into amino acids. Fifty-six patterns of deduced amino acid sequences were obtained without a consensus sequence. Most sequences of mimetic peptides have amino acid motif as RXXR with approximate molecular weight of 1,700 to 2,000. Arginine and glycine occupy the highest percentage of amino acid composition.

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Published

2006-10-30

How to Cite

Aree Thattiyaphong, Amornrat Promboon, Chaivat Kittigul, Krongkaew Supawat, Pathom Sawanpunyalert, and Sunanta Ratanapo. 2006. “Antibody-Binding Motif of Mimetic Peptides to V. Cholerae O139 Lipopolysaccharide”. Agriculture and Natural Resources 40 (5). Bangkok, Thailand:90-95. https://li01.tci-thaijo.org/index.php/anres/article/view/243929.

Issue

Vol. 40 No. 5 (2006): September-October

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.