Nucleotide and Derived Amino Acid Sequences of the Cyanogenic Beta-Glucosidase (Linamarase) from Cassava (Manihot esculenta Crantz)

Authors

  • Prachumporn Toonkool Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
  • Nusra Tongtubtim Department of Biochemistry, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

beta-glucosidase, linamarase, cassava, nucleotide sequence, amino acid sequence

Abstract

Many isozymes of cassava cyanogenic β-glucosidase (linamarase) exist, but only one cDNA sequence (pCAS5) has been reported thus far. In order to study the structure-function relationships in this enzyme, the cDNA of cassava linamarase was cloned and sequenced. In this report, six different
cDNA sequences of linamarase from cassava were cloned by reverse transcription-polymerase chain reaction (RT-PCR) using primers designed from the sequence of pCAS5. Nucleotide sequences of all six clones showed 98-99% sequence identity to the nucleotide sequence of pCAS5. Derived amino acid sequences from four cDNA clones showed 98-99% sequence identity to that of pCAS5, while the other two cDNA clones contained nucleotide sequences that led to premature termination. 

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Published

2006-10-30

How to Cite

Prachumporn Toonkool, and Nusra Tongtubtim. 2006. “Nucleotide and Derived Amino Acid Sequences of the Cyanogenic Beta-Glucosidase (Linamarase) from Cassava (Manihot Esculenta Crantz)”. Agriculture and Natural Resources 40 (5). Bangkok, Thailand:123-28. https://li01.tci-thaijo.org/index.php/anres/article/view/243934.

Issue

Vol. 40 No. 5 (2006): September-October

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.