Isolation, Screening and Identification of Mannanase Producing Microorganisms

Authors

  • Khampheng Phothichitto Research Institute of Science, Prime Minister’s Office, P.O Box 2279, Vientiane, Lao PDR.
  • Sunee Nitisinprasert Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Suttipun Keawsompong Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.

Keywords:

prebiotic, mannan, copra meal, probiotic, locust bean gum

Abstract

Mannan is used in many industries and known to be the major polysaccharides of legume seeds, coconut kernel and tubers of konjac. Several bacteria and fungi have been reported to produce mannanase. In this paper, 19 bacteria and 4 fungi showing mannanase production were isolated from twenty-three soil samples. Isolate NT 6.7 showed high mannanase activity (0.306 unit/ml), displayed broad inhibition to pathogens; Salmonella serovar Enteritidis S003 and Escherichia coli E010, and could promote growth of probiotic Lactobacillus reuteriAC5. The strain was identified by morphological, physiological, biochemical (API 50 CHB, 99.5%identity) and 16 S rDNA sequence (99%identity) tests as Bacillus circulans. The mannanase activity from this strain had optimum pH of 7.0-9.0, optimum temperature at 50 oC and high stability at 50 oC. The results suggested mannanase produced from B. circulans NT 6.7 had interesting properties and could be applied in prebiotic preparation.

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Published

2006-12-30

How to Cite

Khampheng Phothichitto, Sunee Nitisinprasert, and Suttipun Keawsompong. 2006. “Isolation, Screening and Identification of Mannanase Producing Microorganisms”. Agriculture and Natural Resources 40 (6 (Suppl.). Bangkok, Thailand:26-38. https://li01.tci-thaijo.org/index.php/anres/article/view/244012.

Issue

Vol. 40 No. 6 (Suppl.) (2006): November-December

Section

Research Article

License

online 2452-316X print 2468-1458/Copyright © 2022. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/),
production and hosting by Kasetsart University of Research and Development Institute on behalf of Kasetsart University.