Identification of Major Saponins from Jiaogulan Extract (Gynostemma pentaphyllum)

Authors

  • Niramon Utama-ang Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Penkwan Chompreeda Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Vichai Haruthaithanasan Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Nusiri Lerdvuthisopon Faculty of Medicine, Thamasart University, Phathum Thani, Thailand.
  • Thongchai Suwonsichon Department of Product Development, Faculty of Agro-Industry, Kasetsart University, Bangkok 10900, Thailand.
  • Karl Wood Campus-Wide Mass Spectrometry Center, Purdue University, West Lafayette,IN 47907.
  • Bruce A. Watkins Center of Enhancing Foods to Protect Health, Department of Food Science, Purdue University, West Lafayette,IN 47907.

Keywords:

Gynostemma pentaphyllum, Jiaogulan, saponin, Gas chromatography mass spectrophotometry

Abstract

Jiaogulan (Gynostemma pentaphyllum; GP) contained a saponin glycoside which was related to ginseng saponin. The purpose of this study was to identify the main saponin in water extract GP compared to methanol and ethanol extract. The sample was prepared by alkaline cleavage, trimethylsilyl (TMS) derivatization and gas chromatographic-mass spectrometric (GC-MS) analysis comparison with standard ginsenoside Rb1. The results showed that there were two main dammarant type aglycones in water, methanol and ethanol extracts GP, 20(S)-dammar-24-ene-3β, 12β, 20 triol and 20(S)-dammar-24-ene-3β, 6α, 12β, 20 tetrol, which were the same compound as ginsenoside Rb1 and Rg1 from ginseng (Panax spp.), representatively. The other peak, 20(S)-dammar-24-ene-3β, 12β, 19, 20 tetrol, was not obvious in water extract as in methanol and ethanol extracts. In conclusion, the water extract GP contained two main saponin compounds, which were the same compounds as in ginseng. 

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Published

2006-12-30