Production of Fish Serum Products as Substitute for Fetal Bovine Serum in Hybridoma Cell Cultures from Surimi Industrial Waste

Abstract

The hybridoma cell culture procedures mostly utilize fetal bovine serum (FBS) to supplement the culture media, which contributes to the high cost of production of monoclonal antibody. Previous research has shown interesting results on the utilization of fish serum (FS) prepared from wash water of surimi processing line. In this study, FS was used as a substitute for FBS in two types of hybridoma cell cultures. Cell viability was measured using MTT colorant while IgG monoclonal antibody secretion was measured using ELISA method. SDS electrophoresis was run to observe FS protein profiles. Nitrogenous compounds of FS were also determined. Based on the results, it was found that FS prepared from surimi factory wash water contained proteins with molecular weights ranging from 66 to 12 kD. The serum contained TVN and TMA, signalling the need to improve the washing process in the factory, particulary the waste water treatment in formulating the fish serum. The two hybridomas secreting IgG anti-chikungunya (105) and anti-dengue virus (H2) grew differently in the FS-substituted medium, where the 105 hybridoma showed less cell growth. The cells in all substituted cultures produced antibody until the third passage; however, substitution of 2% FBS with FS resulted in the higher antibody secretion, especially in cultures maintained for 96 hours after the third passage.